The assessment of infant immune responses to vaccines is just one of the pressing applications for next-generation spectral flow cytometry. Technical and methodological improvements now allow unprecedented choice in designing high-parameter panels to characterize low-volume samples. This webcast will discuss how new dyes, unmixing algorithms and imaging capabilities are advancing research in the field of paediatrics and maternal-infant health.
The speaker will describe how the new generation of dyes, coupled with optimizing large spectral panels, improves detection of antigen specific T-cell responses in infants, allowing for a creative and multifaceted panel that could enable the most comprehensive assessment of rotavirus vaccine T-cell immunity in 6-month-old infants to date – including T-cell functionality, antigen-induced memory recall, phenotype and T-helper skewing, all within a single assay.
In addition, new work with imaging-coupled spectral cytometry aims to advance the field of breast milk immunology, improving the detection of immune cells within breast milk samples.
You will learn:
• How spectral flow cytometry can improve characterization of immune cells in low-biomass samples
• How new dyes and technologies can improve on existing work
• How infant vaccine responses are shaped by antigen availability and pre-existing immunity
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This webcast has been produced by BD Biosciences, who retains sole responsibility for content. About this content.
Speaker
Alexander Nicols, Postdoctoral Researcher, Pediatric Immunization Advancement Lab, University of California, San Diego
Alexander Nicols, PhD, is a postdoctoral researcher in the Pediatric Immunization Advancement lab at UC San Diego, with expertise in high parameter cytometry and T-cell immunology. His current research interests include improving the understanding of age-dependent differences in immune responses to vaccines and infant T-cell development.
Moderator
Nikki Forrester, Freelance Science Writer and Editor
Nikki Forrester is a science journalist who covers biology, natural history, climate, and the culture of academic research. She earned a PhD in ecology and evolutionary biology in 2019.